mouse monoclonal anti-glun2a (Absolute Biotech Inc)
90
Structured Review
Absolute Biotech Inc
mouse monoclonal anti-glun2a
Mouse Monoclonal Anti Glun2a, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-glun2a/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
Mouse Monoclonal Anti Glun2a, supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse monoclonal anti-glun2a/product/Absolute Biotech Inc
Average 90 stars, based on 1 article reviews
mouse monoclonal anti-glun2a - by Bioz Stars,
2026-02
90/100 stars
Images
1) Product Images from "NMDA Receptors Are Upregulated and Trafficked to the Plasma Membrane after Sigma-1 Receptor Activation in the Rat Hippocampus"
Article Title: NMDA Receptors Are Upregulated and Trafficked to the Plasma Membrane after Sigma-1 Receptor Activation in the Rat Hippocampus
Journal: The Journal of Neuroscience
doi: 10.1523/JNEUROSCI.0458-14.2014
Figure Legend Snippet: Change in protein expression of NMDARs, AMPARs, and PSD-95 after SKF or PTZ administration
Techniques Used: Expressing
Figure Legend Snippet: Injection of σ-1R agonists leads to an increase in GluN2 subunits and PSD-95 expression. A significant increase in protein expression of GluN2A, GluN2B, and PSD-95 was observed 90 min after intraperitoneal injection of vehicle (Veh) or SKF (A, B). Similar results were obtained 90 min after injection of PRE (C) or PTZ (D). There was no change in the expression level of AMPARs after SKF (E, F), PRE (G), or PTZ (H) injection. Bar graphs are mean ± SEM of at least five animals. *p < 0.05.
Techniques Used: Injection, Expressing
Figure Legend Snippet: The increase in NMDAR subunit and PSD-95 expression is also observed after σ-1R activation in hippocampal slices. A, WBs showing the protein level of GluN1, GluN2A, GluN2B, and PSD-95 after a 90 min bath application of 1 or 5 μm SKF. B, A significant increase in GluN1, GluN2A, and GluN2B expression (relative to control; Ctrl) was observed in the presence of 1 μm SKF. A significant increase in PSD-95 expression was observed after application of 5 μm SKF. C, D, The increase in NMDAR and PSD-95 protein levels was abolished after pretreatment with 50 μm BD1063. Bar graphs are mean ± SEM of slices from at least five animals per treatment.
Techniques Used: Expressing, Activation Assay
Figure Legend Snippet: Both acute and chronic administration of σ-1R antagonists block the increase in GluN2 subunit and PSD-95 observed after SKF, PTZ, or PRE administration
Techniques Used: Blocking Assay
Figure Legend Snippet: Chronic and acute administration of BD1047 abolishes the σ-1R-mediated increase in NMDAR subunit and PSD-95 expression in vivo. A, B, There was no change in the expression level of GluN2A, GluN2B, or PSD-95 after a 2 d chronic administration or a single intraperitoneal injection (acute administration) of vehicle (Veh) or the σ-1R antagonist BD1047 (BD). C, D, Chronic administration of BD1047 blocked the increase in GluN2 subunit and PSD-95 levels observed 90 min after an intraperitoneal injection of SKF or PTZ. E, F, Acute administration of BD1047 also abolished the increase in GluN2 subunits and PSD-95 expression after administration of SKF or PRE. Bar graphs are mean ± SEM of at least five animals.
Techniques Used: Expressing, In Vivo, Injection
Figure Legend Snippet: The increase in GluN2 subunits and PSD-95 expression after σ-1R activation is protein synthesis dependent. A, Representative WBs showing that the expression levels of GluN2A, GluN2B, and PSD-95 were unaffected by intraperitoneal injection of vehicle (Veh) or anisomycin (Aniso). However, there was a decrease in the expression level of c-Fos (B), demonstrating that anisomycin blocked protein synthesis at this time point. Interestingly, anisomycin treatment before SKF (C, D) or PTZ (E, F) administration prevented any change in expression levels of GluN2 subunits and PSD-95. Bar graphs are mean ± SEM of at least five animals. *p < 0.05.
Techniques Used: Expressing, Activation Assay, Injection
Figure Legend Snippet: An increase in interaction between σ-1Rs and NMDARs was observed after SKF injection. A, Representative blots showing the specificity of the σ-1R antibody. Single σ-1Rs band detected in rat brain homogenate was abolished in the presence of blocking peptide (A, left). Similarly, the single σ-1Rs band in wild-type mouse hippocampus was not detected in σ-1R−/− mice (A, right). B, Control Co-IP experiment demonstrating that σ-1Rs were immunoprecipitated and detected with the σ-1R antibody. C, Representative WBs from a Co-IP experiment investigating the interaction between σ-1Rs and GluN2 subunits, demonstrating an increased interaction after SKF administration compared with vehicle (Veh). D, Pooled data showing a significant increase in interaction between σ-1Rs and GluN2 subunits after SKF injection. E, Representative WBs from an additional experiment investigating the interaction between PSD-95 and GluN2 subunits after SKF administration. F, The binding of PSD-95 with GluN2A was unaffected by SKF; interestingly, a significant decrease in the interaction between PSD-95 and GluN2B was observed. Bar graphs are mean ± SEM of at least five animals. *p < 0.05.
Techniques Used: Injection, Blocking Assay, Co-Immunoprecipitation Assay, Immunoprecipitation, Binding Assay
Figure Legend Snippet: A redistribution of GluN2 subunits and PSD-95 was observed after administration of SKF. A, Schematic diagram showing the three fractions isolated from LP1 by discontinuous sucrose gradient. B, Representative WBs showing protein expression in the different fractions after injection of vehicle (Veh) or SKF with β-tubulin as a loading control. A significant increase in GluN2A (C) and GluN2B (D) expression was observed in the VF and a significant increase in PSD-95 (E) in both VF and SMF after SKF administration. There is a decrease in expression in the PF for all three proteins investigated (C–E). Bar graphs are mean ± SEM of at least four animals. *p < 0.05.
Techniques Used: Isolation, Expressing, Injection